Signalling

Part:BBa_K1369004:Design

Designed by: Andrew Hou   Group: iGEM14_Caltech   (2014-09-04)


AgrA+SH3 peptide; P2 promoter+GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 289
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 1011
    Illegal BglII site found at 312
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 73
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1946
    Illegal SapI site found at 55


Design Notes

A double terminator sequence separates the AgrA sequence from the P2 promoter sequence.


Source

The AgrA response regulator and P2 promoter are endogenous to Staphylococcus aureus, which are involved in the AgrBDCA quorum sensing system.

References